Cells or organoids in multi-well plates are protected by layers of alginate gel. An alginate solution is pipetted into each well, followed by the gelation solution. After waiting a few minutes for the gel to form, the plates are ready for storage or shipment to the end user.
Adding the release buffer to the wells causes the gel to dissolve. The solutions are then discarded and replaced with your cells’ preferred media and the plates are ready for use.